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Cloning and Expression
Enzymes
BssHII
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BssHII
BssHII from New England Biolabs
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Citations:
(16)
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New England Biolabs for
BssHII
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
BssHII
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0199L
Quantity
2500 units
Concentration
4-20 u/µL
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
G/CGCGC
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (16)
(1)
Rapid and Quantitative Functional Interrogation of Human Enhancer Variant Activity in Live Mice
bioRxiv
December 10, 2023
Ethan W. Hollingsworth, Taryn A. Liu, Sandra H. Jacinto, Cindy X. Chen, Joshua A. Alcantara, Evgeny Z. Kvon
via
PCR
. For dual-enSERT-2.2, a dual-enSERT-2.1 plasmid (1 μg) was digested overnight with PauI (
NEB
,
R0199
) at 50°C in
rCutSmart Buffer
. The following day, the reaction was inactivated by
incubation
at 65°C
(2)
Cell type specializations of the vocal-motor cortex in songbirds
Cell Reports
November 28, 2023
Alexander A. Nevue, Benjamin M. Zemel, Samantha R. Friedrich, Henrique von Gersdorff, Claudio V. Mello
DIG RNA Labeling Mix
,
Roche
,Cat#
11277073910
Triethanolamine,Fisher,Cat#T350
Formamide
,
Fisher
,Cat#F84
BCIP/NBT
,
Perkin-Elmer
,Cat#NEL937 Acetic Anhydride,Sigma,Cat#A6404
Triton X-100
,
Sigma
,Cat#T8787
BSSHII
,
NEB
,Cat#
R0199S
T3 RNA Polymerase
,
Promega
,Cat#P208C Tyramide conjugates,Invitrogen,Cat#B40952; B40953 CUBIC reagent,Lee
(3)
Identification of an autotransporter peptidase of Rickettsia rickettsii responsible for maturation of surface exposed autotransporters
PLOS Pathogens
July 31, 2023
Adam M. Nock, Karin Aistleitner, Tina R. Clark, Dan Sturdevant, Stacy Ricklefs, Kimmo Virtaneva, Yixiang Zhang, Naila Gulzar, Neelam Redekar, Amitiva Roy,
et al.
GeneJet PCR Purification Kit
(
Thermoscientific
,
K0702
), digested with
BsiWi-HF
(
NEB
,
R3553S
) and
BssHII
(
NEB
,
R0199S
), then re-purified using the GeneJet PCR Purification Kit per manufacturer’s instructions. Plasmids
(4)
Simultaneous affinity maturation and developability enhancement using natural liability-free CDRs
mAbs
December 31, 2022
Andre A. R. Teixeira et al.
with 4 μg of the
yeast
display vector pSYD previously digested with the enzymes
BssHII
and
NheI
(
NEB
#
R0199S
and #
R0131S
) by
electroporation
using the method described previously.71 Phase 2 library combo construction For
(5)
Multiplexed promoter and gene editing in wheat using a virusâ€based guide RNA delivery system
Plant Biotechnology Journal
September 7, 2022
Wei Wang, Zitong Yu, Fei He, Guihua Bai, Harold N. Trick, Alina Akhunova, Eduard Akhunov
expressing BSMV γ chain carrying sgRNAs were linearized with
BssHII
(
New England BioLabs
, catalogue number
R0199S
).
In vitro transcription
was performed in 20 μL reaction volume using
HiScribe™ T7 High Yield RNA Synthesis Kit
(6)
Emergence of sex-specific transcriptomes in a sexually dimorphic brain nucleus
Cell Reports
August 2, 2022
Samantha R. Friedrich, Alexander A. Nevue, Abraão L.P. Andrade, Tarciso A.F. Velho, Claudio V. Mello
DIG RNA Labeling Mix
,
Roche
,Cat#
11277073910
Triethanolamine,Fisher,Cat#T350
Formamide
,
Fisher
,Cat#F84
BCIP/NBT
,
Perkin-Elmer
,Cat#NEL937 Acetic Anhydride,Sigma,Cat#A6404
Triton X-100
,
Sigma
,Cat#T8787
BSSHII
,
NEB
,Cat#
R0199S
T3 RNA Polymerase
,
Promega
,Cat#P208C ,, Critical commercial
assays
,, ,, RNeasy Micro Kit,
Qiagen
,Cat#
74004
RNA
(7)
Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model
Cell reports
May 31, 2022
Cheng Y, Medina A, Yao Z, Basu M, Natekar JP, Lang J, Sanchez E, Nkembo MB, Xu C, Qian X,
et al
.
Sp6 RNA polymerase
,
NEB
,Cat#
M0207S
SacI-HF
,
NEB
,Cat#
R3156S
ClaI
,
NEB
,Cat#
R0197S
AatII
,
NEB
,Cat#
R0117S
BsHII,
NEB
,Cat#
R0199S
Human brain microvascular endothelial cell lysate,
ScienCell
,Cat#
1006
Sodium Citrate Dihydrate,Fisher
(8)
Healthy humans can be a source of antibodies countering COVID-19
Bioengineered
May 1, 2022
Velappan N, Nguyen HB, Micheva-Viteva S, Bedinger D, Ye C, Mangadu B, Watts AJ, Meagher R, Bradfute S, Hu B,
et al
.
all 18
clones
were excised from PDNL6 plasmids by restriction digestion with enzymes
BssHII
(
NEB
, #
R0199S
) and
NheI
(
NEB
, #
R3131S
) and gel purified. These genes were inserted into a
yeast
scFv-Fc expression
(9)
Cleavage of viral DNA by restriction endonucleases stimulates the type II CRISPR-Cas immune response
Molecular Cell
March 1, 2022
Pascal Maguin, Andrew Varble, Joshua W. Modell, Luciano A. Marraffini
Enzyme,
New England Biolabs
,Catalog No.
R0102
BssHII
Restriction Enzyme,
New England Biolabs
,Catalog No.
R0199
DNase I
,
Sigma
,Catalog No.
AMPD1
-1KT Heart Infusion (HI)
Agar
,Becton Dickson,Catalog No. 244400 Lysostaphin,Ambi
(10)
Protocol: A Multiplexed Reporter Assay to Study Effects of Chromatin Context on DNA Double-Strand Break Repair
Frontiers in Genetics
January 31, 2022
Ruben Schep, Christ Leemans, Eva K. Brinkman, Tom van Schaik, Bas van Steensel
R3131S
/L—20 U/µl)8.
KpnI-HF
® (
New England BioLabs
—Cat#:
R3142S
/L—20 U/µl)9.
BssHII
(
New England BioLabs
—Cat#:
R0199S
/L—5 U/µl)10.
MluI-HF
(
New England BioLabs
—Cat#:
R0198S
/L—20 U/µl)11. Ampicilin (brand not critical)12.
(11)
Drug-like antibodies with high affinity, diversity and developability directly from next-generation antibody libraries
mAbs
December 1, 2021
Andre Azevedo Reis Azevedo Reis Teixeira et al.
used Q5 polymerase. The scFv amplicons were digested using
BssHII
and
NheI
restriction enzymes (
NEB
, #
R0199L
and #
R0131L
) and ligated to the pDAN5 phagemid vector22 previously digested with the same enzymes.
Ligation
(12)
Controlling for activity‐dependent genes and behavioral states is critical for determining brain relationships within and across species
Journal of Comparative Neurology
August 1, 2021
Matthew T. Biegler, Lindsey J. Cantin, Danielle L. Scarano, Erich D. Jarvis
the plasmid with
PvuII‐HF
(
New England Biolabs
, Cat. #R3151) or
BssHII
(
New England Biolabs
, Cat. #
R0199
) restriction enzymes, targeting restriction sites flanking the probe sequence. The cDNA products were
(13)
Construction, characterization and crystal structure of a fluorescent single-chain Fv chimera
Protein Engineering, Design and Selection
February 15, 2021
Nileena Velappan, Devin Close, Li-Wei Hung, Leslie Naranjo, Colin Hemez, Natasha DeVore, Donna K McCullough, Antonietta M Lillo, Geoffrey S Waldo, Andrew R M Bradbury
genes were cloned into the vector systems described below, using restriction enzymes BssH II and Nhe I (
R0199
and R3131,
New England Biolabs
). The scFv and scTGP genes were both cloned into pEP, a periplasmic expression
(14)
Capsid-CPSF6 Interaction Licenses Nuclear HIV-1 Trafficking to Sites of Viral DNA Integration
Cell Host & Microbe
September 1, 2018
Vasudevan Achuthan, Jill M. Perreira, Gregory A. Sowd, Maritza Puray-Chavez, William M. McDougall, Adriana Paulucci-Holthauzen, Xiaolin Wu, Hind J. Fadel, Eric M. Poeschla, Asha S. Multani,
et al.
,
Thermo Fisher Scientific
,Cat #
15630080
T4 DNA Ligase
,
New England Biolabs
,Cat #
M0202L
BssHII
,
New England Biolabs
,Cat #
R0199L
SpeI
,
New England Biolabs
,Cat #
R0133L
MseI
,
New England Biolabs
,Cat #
R0525L
BglII
,
New England Biolabs
,Cat
(15)
Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
mAbs
August 1, 2018
Emanuele Sasso et al.
cloning kit (Clontech Laboratories, 639,692) was used to
clone
the VHs in
BamHI
(
R3136
) and
BssHII
(
R0199
) all from
New England Biolabs
, linearized pEU8.2VH vector, and the VLs in
ApaLI
(
R0507
) and
AvrII
(
R0174
)
(16)
Protein and Antibody Engineering by Phage Display
Methods in Enzymology
September 3, 2016
J.C. Frei, J.R. Lai
catalog #:
M0531S
) Restriction enzymes:
BssHII
,
BsiWI
,
NheI
,
NsiI
,
FseI
(
NEB
, Ipswich, MA, catalog #s:
R0199S
,
R0553S
,
R0131S
,
R0127S
,
R0558S
, respectively) Alkaline Phosphatase, Calf Intestinal (
NEB
, Ipswich,
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BssHII from New England Biolabs
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